5IXM

The LPS Transporter LptDE from Yersinia pestis, core complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	90
Detector technology	CCD
Collection date	2012-07-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	82.892, 176.354, 143.848
Unit cell angles	90.00, 96.11, 90.00

Refinement procedure

Resolution	40.129 - 2.746
R-factor	0.2147
Rwork	0.212
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.998
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.129
High resolution limit [Å]	2.746
Rmerge	0.150
Number of reflections	106219
<I/σ(I)>	8.4
Completeness [%]	99.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	50 mM sodium cacodylate pH 6.5, 10% polyethelyene glycol (PEG) 2000, 15% PEG 6000