5IXI
Structure of human JAK1 FERM/SH2 in complex with IFNLR1/IL10RA chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000000000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.170, 110.420, 94.870 |
Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
Resolution | 36.150 - 2.570 |
R-factor | 0.2166 |
Rwork | 0.213 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ixd |
RMSD bond length | 0.006 |
RMSD bond angle | 1.011 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.150 | 2.660 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.083 | 0.732 |
Number of reflections | 26423 | |
<I/σ(I)> | 13.8 | 2.2 |
Completeness [%] | 99.4 | 98 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES pH6.5, 0.2M MgCl2, 9% PEG4K, 25% ethylene glycol |