5IXC
Human GIVD cytosolic phospholipase A2 in complex with Methyl gamma-Linolenyl Fluorophosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.997648 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.567, 111.379, 157.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.970 - 2.650 |
| R-factor | 0.23732 |
| Rwork | 0.237 |
| R-free | 0.25068 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5izr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.970 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.092 | |
| Number of reflections | 52014 | |
| <I/σ(I)> | 20 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES (pH 6.0-6.2), 50 mM sodium acetate, and 10-14% (v/v) PEG20K |
