5IXA
HCMV DNA polymerase processivity subunit UL44 at neutral pH and low salt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-08-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | .97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.026, 100.829, 109.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.108 - 2.684 |
R-factor | 0.2128 |
Rwork | 0.210 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t6l |
RMSD bond length | 0.011 |
RMSD bond angle | 1.587 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.108 |
High resolution limit [Å] | 2.684 |
Number of reflections | 23307 |
<I/σ(I)> | 10.6 |
Completeness [%] | 98.0 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.2 microliter of 8 mg/mL UL44 in the storage buffer (20 mM Tris (PH 7.5), 500 mM NaCl, 0.1 mM EDTA, 2 mM DTT and 5% glycerol) and 0.2 microliter crystallization buffer (0.1 M HEPES (PH 7.0) and 18% PEG12K) were mixed and crystallized in sitting drops over the crystallization buffer at room temperature |