5IWL
CD47-diabody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.127085 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.280, 103.280, 131.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.722 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | XDS (June 17, 2015) |
| Data scaling software | XDS (June 17, 2015) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.10 r2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 2.562 | |
| Number of reflections | 20405 | |
| <I/σ(I)> | 9.1 | 0.95 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 295 | sodium cacodylate pH 6.0, 17% PEG 4000 |
