5IVX
Crystal Structure of B4.2.3 T-Cell Receptor and H2-Dd P18-I10 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.107, 51.316, 93.741 |
| Unit cell angles | 90.00, 97.14, 90.00 |
Refinement procedure
| Resolution | 29.799 - 2.100 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lp9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.655 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.082 | 0.332 |
| Number of reflections | 57154 | |
| <I/σ(I)> | 16.3 | 2.2 |
| Completeness [%] | 97.8 | 80.1 |
| Redundancy | 6.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 15% PEG 3350, 0.1M HEPES, 0.15M Magnesium Chloride |
