5IVB
A High Resolution Structure of a Linked KDM5A Jmj Domain with Alpha-Ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.340, 62.199, 46.751 |
| Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
| Resolution | 32.883 - 1.389 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e6h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.716 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2356) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.883 | 1.420 |
| High resolution limit [Å] | 1.389 | 1.390 |
| Rmerge | 0.158 | 0.671 |
| Number of reflections | 64066 | |
| <I/σ(I)> | 13.8 | 1 |
| Completeness [%] | 96.1 | 63.9 |
| Redundancy | 10.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 1.2-1.35 M (NH4)2SO4, 0.1 M Tris-HCl (pH 8.6-9.2) 0-20% glycerol 25 mM (Na/K) dibasic/monobasic phosphate |
