5IU9
Crystal Structure of Zebrafish Protocadherin-19 EC1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.390, 59.776, 165.925 |
| Unit cell angles | 90.00, 94.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.590 |
| R-factor | 0.24851 |
| Rwork | 0.246 |
| R-free | 0.30498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5co1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.466 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.660 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Rmerge | 0.386 | |
| Number of reflections | 15416 | |
| <I/σ(I)> | 4.9 | 2.1 |
| Completeness [%] | 94.8 | 86 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 277 | 200 mM Sodium Chloride, 100 mM TrisHCl pH 8.1, 8% PEG 20,000 |
