5ITI
A cynobacterial PP2C (tPphA) structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 38.008, 153.823, 82.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.228 - 1.950 |
R-factor | 0.221 |
Rwork | 0.215 |
R-free | 0.27010 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.942 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155: ???) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.230 |
High resolution limit [Å] | 1.950 |
Number of reflections | 18220 |
<I/σ(I)> | 15.7 |
Completeness [%] | 99.9 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.4 | 298 | 100mM Tris-HCl, pH 7.4, 200mM CaCl2, PEG 3350 |