5ITI

A cynobacterial PP2C (tPphA) structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	38.008, 153.823, 82.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.228 - 1.950
R-factor	0.221
Rwork	0.215
R-free	0.27010
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155: ???)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.230
High resolution limit [Å]	1.950
Number of reflections	18220
<I/σ(I)>	15.7
Completeness [%]	99.9
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.4	298	100mM Tris-HCl, pH 7.4, 200mM CaCl2, PEG 3350