5ISI
Crystal structure of mouse CARM1 in complex with inhibitor SA0920 (5'-amino-5'-deoxyadenosine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.736, 98.522, 207.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.709 - 2.740 |
R-factor | 0.1767 |
Rwork | 0.175 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ih3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.847 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1951) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.740 | 2.740 |
Number of reflections | 39458 | |
<I/σ(I)> | 17.5 | 3.6 |
Completeness [%] | 98.9 | 98.4 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | Tris-HCl pH 8.0 100 mM PEG 2000 MME 16% NaCl 67 mM |