5IRO
Crystal structure of a complex between the Human adenovirus type 4 E3-19K protein and MHC class molecule HLA-A2/TAX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-09-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 3 |
Unit cell lengths | 165.727, 165.727, 122.858 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.625 - 2.640 |
R-factor | 0.2589 |
Rwork | 0.257 |
R-free | 0.28830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | HLA-A2 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.237 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.690 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.170 | 0.619 |
Number of reflections | 110878 | |
<I/σ(I)> | 23.4 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.9 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 291 | 1.5M (NH4)2SO4,0.1M Bis-Tris pH6.5, 0.1MNaCI |