5IRN

Crystal structure of rabbit NOD2 in an ADP-bound state (Crystal form1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	77.815, 106.162, 185.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.700 - 2.340
R-factor	0.20329
Rwork	0.202
R-free	0.23585
RMSD bond length	0.012
RMSD bond angle	1.507
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	92.700
High resolution limit [Å]	2.300
Number of reflections	57510
<I/σ(I)>	29.7
Completeness [%]	92.6
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	5% PEG 20000, 5% MPD, 100 mM Hepes pH 7.8, 500 mM NaCl