5IRM
Crystal structure of rabbit NOD2 in an ADP-bound state (Crystal form2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.153, 122.870, 177.555 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.310 |
R-factor | 0.19511 |
Rwork | 0.193 |
R-free | 0.23681 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.279 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 35238 |
<I/σ(I)> | 8.4 |
Completeness [%] | 100.0 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 5% PEG 10000, 100 mM Hepes pH 7.8, 500 mM NaCl |