EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | OTHER |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 183615 |
| Software | EMAN,RELION,REFMAC,EPU,Gctf,UCSF Chimera,Coot |
| CTF correction | NONE |
