5IP4

X-RAY STRUCTURE OF THE C-TERMINAL DOMAIN OF HUMAN DOUBLECORTIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	89.840, 86.191, 73.267
Unit cell angles	90.00, 123.00, 90.00

Refinement procedure

Resolution	44.770 - 1.810
R-factor	0.1967
Rwork	0.194
R-free	0.24635
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENSEMBLE OF 2X1O 2x1p 2x6m 3dwt 3eak 3g9a AND 3P0G
RMSD bond length	0.019
RMSD bond angle	2.121
Data reduction software	XDS ((VERSION December 6 2010))
Data scaling software	SADABS (2008/1)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.6.0112)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	1.900
High resolution limit [Å]	1.810	1.810
Rmeas	0.077	1.100
Number of reflections	42622
<I/σ(I)>	10.33	1.27
Completeness [%]	99.8	99.7
Redundancy	3.42	3.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	20% PEGMME, 100 mM Bis-Tris pH 6.5