5INN
Mouse Tdp2 D358N protein, apo state with increased disorder amongst variable DNA-binding grasp conformations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.440, 114.880, 115.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.393 - 2.800 |
R-factor | 0.2151 |
Rwork | 0.213 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gz1 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.577 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.113 | 0.052 | 0.459 |
Rmeas | 0.129 | ||
Rpim | 0.061 | ||
Total number of observations | 127542 | ||
Number of reflections | 30546 | ||
<I/σ(I)> | 6.2 | ||
Completeness [%] | 95.7 | 90.8 | 93 |
Redundancy | 4.2 | 4.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 14-18% PEG3350, 100mM HEPES, 200mM lithium sulfate, 10mM magnesium acetate |