5IML

Nanobody targeting human Vsig4 in Spacegroup P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	29.269, 50.048, 160.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.780 - 1.800
R-factor	0.1852
Rwork	0.181
R-free	0.22610
RMSD bond length	0.015
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.780
High resolution limit [Å]	1.800
Number of reflections	22570
<I/σ(I)>	12.79
Completeness [%]	99.5
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	(1) 1.2M sodium citrate tribasic dehydrate, 0.1M BIS-TRIS propane pH7.0 and (2) 0.17M Ammonium sulfate, 15% Glycerol, 25.5% PEG 4000