5ILQ
Crystal structure of truncated unliganded Aspartate Transcarbamoylase from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-29 |
Detector | DECTRIS PILATUS3 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.980, 103.800, 87.110 |
Unit cell angles | 90.00, 117.68, 90.00 |
Refinement procedure
Resolution | 45.030 - 2.500 |
R-factor | 0.2082 |
Rwork | 0.208 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ml4 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.961 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.030 | 2.560 |
High resolution limit [Å] | 2.420 | 2.420 |
Rmerge | 0.062 | 0.580 |
Number of reflections | 45217 | |
<I/σ(I)> | 10.81 | 1.98 |
Completeness [%] | 95.2 | 95.6 |
Redundancy | 2.51 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 200 mM Sodium Sulphate, 5 mM MgSO4, 15-18 % (w/v) PEG 3350 in 100mM bis-Tris Propane pH 7.5 |