5ILO

Crystal structure of photoreceptor dehydrogenase from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	C 1 2 1
Unit cell lengths	70.159, 62.646, 114.113
Unit cell angles	90.00, 96.26, 90.00

Refinement procedure

Resolution	113.430 - 2.710
R-factor	0.2063
Rwork	0.204
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b2l
RMSD bond length	0.003
RMSD bond angle	0.744
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.430	2.840
High resolution limit [Å]	2.710	2.710
Rmerge	0.163	0.655
Number of reflections	13342
<I/σ(I)>	9.53	2.4
Completeness [%]	98.5	89.1
Redundancy	3.8	3.7
CC(1/2)	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	PEG 3350, beta- ionone