5IK2
Caldalaklibacillus thermarum F1-ATPase (epsilon mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87260 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 148.240, 131.290, 212.010 |
Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
Resolution | 201.400 - 2.600 |
R-factor | 0.2138 |
Rwork | 0.213 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hkk |
RMSD bond length | 0.007 |
RMSD bond angle | 1.057 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.1.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 201.400 | 46.940 | 2.640 |
High resolution limit [Å] | 2.600 | 14.240 | 2.600 |
Rmerge | 0.056 | 0.014 | 0.273 |
Number of reflections | 220174 | ||
<I/σ(I)> | 10.1 | ||
Completeness [%] | 93.9 | 85.2 | 87.2 |
Redundancy | 1.7 | 1.9 | 1.6 |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.6 | 295 | PEG 4600, Bis-Tris, Tris-HCl, glycerol, MgCl2, ADP, NaCl |