5IJZ

Crystal structure of glutamate dehydrogenase(GDH) from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	171.219, 93.032, 187.875
Unit cell angles	90.00, 108.16, 90.00

Refinement procedure

Resolution	34.366 - 2.290
R-factor	0.1674
Rwork	0.165
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bgv
RMSD bond length	0.015
RMSD bond angle	1.789
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	178.510	2.340
High resolution limit [Å]	2.290	2.300
Number of reflections	249267
<I/σ(I)>	21.14
Completeness [%]	98.6
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG 3350, Tacsimate