5IJZ
Crystal structure of glutamate dehydrogenase(GDH) from Corynebacterium glutamicum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-23 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 171.219, 93.032, 187.875 |
Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
Resolution | 34.366 - 2.290 |
R-factor | 0.1674 |
Rwork | 0.165 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bgv |
RMSD bond length | 0.015 |
RMSD bond angle | 1.789 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 178.510 | 2.340 |
High resolution limit [Å] | 2.290 | 2.300 |
Number of reflections | 249267 | |
<I/σ(I)> | 21.14 | |
Completeness [%] | 98.6 | |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 3350, Tacsimate |