5IJE
Crystal structure of Equine Serum Albumin in the presence of 30 mM zinc at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 61 |
| Unit cell lengths | 94.384, 94.384, 141.905 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.400 |
| R-factor | 0.1985 |
| Rwork | 0.195 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iih |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.204 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.010 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.079 | 0.045 | 0.707 |
| Rmeas | 0.090 | 0.052 | 0.807 |
| Rpim | 0.042 | 0.025 | 0.383 |
| Total number of observations | 120428 | ||
| Number of reflections | 27885 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 99.5 | 94.9 | 99.9 |
| Redundancy | 4.3 | 4.1 | 4.1 |
| CC(1/2) | 0.997 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 1 ul of 30 mg/ml protein in 10 mM Tris pH 7.5 and 150 mM NaCl buffer was mixed with 1 ul of the well condition (0.2 M Li2SO4, 0.1 M Tris:HCl, 2.0 M (NH4)2SO4, 5 mM ZnCl2, final pH 7.4) and equilibrated against well solution in 15 Well Crystallization Plate (Qiagen). Crystals were soaked with 100 mM ZnCl2 in 100 mM Tris, final pH 7.4, to final concentration of 100 mM |
