Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.752, 148.559, 131.720 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 42.360 - 2.570 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ijb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.595 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 6.920 | 2.550 |
| Rmerge | 0.104 | 0.056 | 0.959 |
| Rmeas | 0.116 | 0.066 | |
| Rpim | 0.063 | 0.035 | 0.606 |
| Total number of observations | 315421 | ||
| Number of reflections | 84084 | 4255 | 4086 |
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.6 | 98.6 | 97.5 |
| Redundancy | 3.8 | 3.7 | 3.3 |
| CC(1/2) | 0.991 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris, pH 8.0, 0.2 M lithium chloride, 12% PEG8000 |
