5IIO
Crystal structure of the DNA polymerase lambda binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 191.423, 98.980, 105.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.086 - 2.080 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.23510 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.090 |
High resolution limit [Å] | 2.080 |
Number of reflections | 118537 |
<I/σ(I)> | 20 |
Completeness [%] | 98.2 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M KCl, 0.01 M CaCl2, 0.05 M HEPES pH 7.0, and 10% PEG 400 |