5IHE
D-family DNA polymerase - DP1 subunit (3'-5' proof-reading exonuclease)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-29 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.969, 91.415, 145.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.420 - 2.500 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.22480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.300 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 39885 | |
<I/σ(I)> | 6.9 | |
Completeness [%] | 98.9 | |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 100mM Na cacodylate pH6.7 200mM Ca Acetate 5% PEG8000 |