5IH3
Crystal structure of mouse CARM1 in complex with SAH at 1.8 Angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9304 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.628, 97.920, 204.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.620 - 1.770 |
| R-factor | 0.1653 |
| Rwork | 0.164 |
| R-free | 0.19050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b3f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (dev_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.129 | 1.396 |
| Number of reflections | 146611 | |
| <I/σ(I)> | 11.2 | 1.5 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 7.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | Tris-HCl pH 8.0 100 mM, PEG 2000 MME 19%, NaCl 167 mM |
