5IH1

Macrolide 2'-phosphotransferase type II - complex with GDP and phosphorylated josamycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-12
Detector	RAYONIX MX-300
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	40.370, 80.870, 97.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.867 - 1.310
R-factor	0.1615
Rwork	0.161
R-free	0.17810
RMSD bond length	0.009
RMSD bond angle	1.357
Data scaling software	XSCALE
Refinement software	PHENIX (dev_1627)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.113		1.340
High resolution limit [Å]	1.310	5.860	1.310
Rmerge	0.046	0.041	0.661
Number of reflections	77829
<I/σ(I)>	21.66	48.26	3.1
Completeness [%]	100.0	99.7	100
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	0.1 M calcium acetate, 0.1M Tris, 25-40% PEG 4000