5IGY

Macrolide 2'-phosphotransferase type II - complex with GDP and erythromycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-23
Detector	RAYONIX MX-300
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	39.870, 80.520, 97.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.818 - 1.450
R-factor	0.1714
Rwork	0.170
R-free	0.19580
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5igw
RMSD bond length	0.009
RMSD bond angle	1.302
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.818		1.490
High resolution limit [Å]	1.450	6.480	1.450
Rmerge	0.053	0.028	0.758
Number of reflections	56698
<I/σ(I)>	25.55	61.08	2.85
Completeness [%]	100.0	98.2	99.9
Redundancy	8.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	0.2 M calcium acetate, 0.1M Tris, 25-40% PEG 4000