5IGX
Crystal structure of NIH45-46 Fab germline precursor in complex with 426c.TM1deltaV1-3 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9541 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 146.284, 168.533, 176.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.167 - 3.390 |
| R-factor | 0.2801 |
| Rwork | 0.280 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jdv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.453 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.170 | 39.170 | 3.660 |
| High resolution limit [Å] | 3.390 | 8.970 | 3.390 |
| Rmerge | 0.120 | 0.032 | 0.591 |
| Rmeas | 0.125 | ||
| Rpim | 0.034 | ||
| Total number of observations | 204811 | ||
| Number of reflections | 15282 | ||
| <I/σ(I)> | 15.8 | ||
| Completeness [%] | 99.7 | 99 | 99.1 |
| Redundancy | 13.4 | 12.7 | 13.2 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M sodium citrate pH 5.5, 22% (w/v) PEG 1,000, 3% (w/v) xylitol |
