5IFM
Human NONO (p54nrb) Homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.826 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.150, 407.177, 68.961 |
| Unit cell angles | 90.00, 97.75, 90.00 |
Refinement procedure
| Resolution | 48.150 - 2.600 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ca5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 | 0.742 |
| Number of reflections | 110370 | |
| <I/σ(I)> | 10.1 | 1.6 |
| Completeness [%] | 98.9 | 97.7 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M HEPES pH 8.0 and 20% w/v PEG 3350 |
