5IFK
Purine nucleoside phosphorylase
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 61 |
| Unit cell lengths | 80.780, 80.780, 231.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.787 - 1.967 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.076 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 231.161 | 39.787 | 2.070 |
| High resolution limit [Å] | 1.967 | 6.220 | 1.970 |
| Rmerge | 0.023 | 0.340 | |
| Number of reflections | 60087 | ||
| <I/σ(I)> | 16 | 25.7 | 2.2 |
| Completeness [%] | 99.7 | 98.8 | 98.6 |
| Redundancy | 4.8 | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1M sodium acetate trihydrate, 8% PEG 4000, pH 4.6 |
