5IEF
Murine endoplasmic reticulum alpha-glucosidase II with N-butyl-1-deoxynojirimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 103.840, 172.890, 62.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.720 - 2.380 |
R-factor | 0.1875 |
Rwork | 0.186 |
R-free | 0.21760 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5f0e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 103.840 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Number of reflections | 44500 | |
<I/σ(I)> | 5.6 | 0.9 |
Completeness [%] | 95.9 | 99.8 |
Redundancy | 4.9 | 5 |
CC(1/2) | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 21% v/v ethylene glycol, 11% w/v PEG 8000 (from the Morpheus Precipitant Mix 2), 50 mM Morpheus carboxylic acids mix, 100 mM Morpheus buffer system 1 pH 6.25 |