5ICW

Crystal structure of human NatF (hNaa60) homodimer bound to Coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	63.840, 84.298, 68.485
Unit cell angles	90.00, 91.10, 90.00

Refinement procedure

Resolution	46.249 - 1.951
R-factor	0.2095
Rwork	0.207
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lx9
RMSD bond length	0.008
RMSD bond angle	1.278
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.458	1.989
High resolution limit [Å]	1.951	1.951
Rmerge	0.098
Number of reflections	52859
<I/σ(I)>	12.82
Completeness [%]	94.6
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	3% Tascimate, 0.1 M Bistris pH 6.5 and 16% PEG 3350