5IC0
Structural analysis of a talin triple domain module
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2012-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.072 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.007, 77.573, 61.622 |
Unit cell angles | 90.00, 109.82, 90.00 |
Refinement procedure
Resolution | 46.440 - 1.970 |
R-factor | 0.20275 |
Rwork | 0.201 |
R-free | 0.23043 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w8p 4f7g |
RMSD bond length | 0.006 |
RMSD bond angle | 0.934 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 46.440 |
High resolution limit [Å] | 1.970 |
Number of reflections | 29840 |
<I/σ(I)> | 13.2 |
Completeness [%] | 99.3 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH6.5, 21% (w/v) PEG 3350, 2% (w/v) benzamidine hydrochloride |