5I9W
Crystal Structure of Ephrin A2 (EphA2) Receptor Protein Kinase with ANP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.733, 107.547, 40.479 |
| Unit cell angles | 90.00, 108.77, 90.00 |
Refinement procedure
| Resolution | 38.326 - 1.359 |
| R-factor | 0.1702 |
| Rwork | 0.170 |
| R-free | 0.17960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mqb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.330 | 1.440 |
| High resolution limit [Å] | 1.359 | 1.359 |
| Rpim | 0.095 | 0.679 |
| Number of reflections | 56636 | |
| <I/σ(I)> | 13.04 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30 % MPD_P1k_P3350, 0.1 M AminoAcidsMix, 0.1 M HEPES pH 7.0 |
