5I7Z

Crystal structure of a Par-6 PDZ-Crumbs 3 C-terminal peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	65.060, 65.060, 52.596
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.448 - 1.801
R-factor	0.1987
Rwork	0.197
R-free	0.21770
RMSD bond length	0.015
RMSD bond angle	1.324
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.073	0.418
Number of reflections	10639
<I/σ(I)>	43.4	1.9
Completeness [%]	95.6	70.6
Redundancy	12.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	288	26% PEG 6000, 100 mM HEPES