5I6Z
X-ray structure of the ts2 human serotonin transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 129.750, 162.810, 140.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 4.530 |
| R-factor | 0.282 |
| Rwork | 0.280 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.498 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.500 |
| High resolution limit [Å] | 4.530 |
| Rmerge | 0.186 |
| Number of reflections | 17307 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 99.4 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Tris pH 8, 250 mM LiCl, 34.7% PEG 400 |
