5I6C
The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.992 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.090, 173.830, 82.390 |
Unit cell angles | 90.00, 111.11, 90.00 |
Refinement procedure
Resolution | 76.861 - 3.700 |
R-factor | 0.2978 |
Rwork | 0.296 |
R-free | 0.32670 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.103 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.900 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.102 | 0.965 |
Number of reflections | 20599 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 97.8 | |
Redundancy | 2.5 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | Peg300, 0.1 MES ph 6.5 |