5I68
Alcohol oxidase from Pichia pastoris
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
EM Data Collection
| Microscope | JEOL 3200FSC |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 4.2 |
| Energy Filter | In-column Omega Filter |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.37 |
| Number of Particle | 56544 |
| Software | EMAN,RELION,Coot,PHENIX,UCSF Chimera |
| Symmetry | D4 |
| CTF correction | PHASE FLIPPING ONLY |
