5I61

Crystal structure of the RNA-dependent RNA polymerase of a human picorbirnavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	77
Detector technology	CMOS
Collection date	2011-11-21
Detector	NOIR-1
Wavelength(s)	0.9785
Spacegroup name	P 1 21 1
Unit cell lengths	60.756, 78.763, 101.449
Unit cell angles	90.00, 91.44, 90.00

Refinement procedure

Resolution	43.795 - 2.400
R-factor	0.1725
Rwork	0.170
R-free	0.22390
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.310
High resolution limit [Å]	2.270	6.160	2.270
Rmerge	0.113	0.066	0.481
Rmeas	0.132	0.077	0.593
Rpim	0.067	0.039	0.342
Total number of observations	165286
Number of reflections	44254
<I/σ(I)>	7.7
Completeness [%]	99.3	100	90.4
Redundancy	3.7	3.7	2.7
CC(1/2)		0.996	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	200 mM sodium acetate, 100 mM sodium cacodylate pH 6.5, and 30% PEG 8000