5I4R
Contact-dependent inhibition system from Escherichia coli NC101 - ternary CdiA/CdiI/EF-Tu complex (trypsin-modified)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.395, 128.354, 100.358 |
| Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.24399 |
| Rwork | 0.243 |
| R-free | 0.26778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i4q 1erc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.118 | 0.959 |
| Number of reflections | 26374 | |
| <I/σ(I)> | 10.6 | 1.5 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.05 M KCl 0.1 M HEPES pH=7.0, 1.0 M ammonium sulfate, cryo 3.0 M ammonium sulfate |
