5I4H
Caught in the Act: The Crystal Structure of cleaved Cathepsin L bound to the active site of Cathepsin L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.974, 67.369, 77.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.700 - 1.420 |
| Rwork | 0.163 |
| R-free | 0.18410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu3 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.6) |
| Refinement software | MAIN |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.470 | |
| High resolution limit [Å] | 1.419 | 4.240 | 1.419 |
| Rmerge | 0.053 | 0.022 | 0.437 |
| Number of reflections | 38421 | ||
| <I/σ(I)> | 20.46 | 70.26 | 2.56 |
| Completeness [%] | 95.4 | 99.6 | 69.23 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.11 M HEPES, pH 7.5, 2.14 M ammonium sulfate |
