5I2C
Arginine-bound CASTOR1 from Homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.393, 82.601, 96.666 |
Unit cell angles | 90.00, 116.23, 90.00 |
Refinement procedure
Resolution | 86.712 - 1.801 |
R-factor | 0.1723 |
Rwork | 0.172 |
R-free | 0.20440 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.846 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.720 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 116806 | |
<I/σ(I)> | 25.9 | |
Completeness [%] | 97.8 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.15 | 0.1 M sodium acetate, 0.25 M ammonium acetate, 22.5% PEG 3350 |