5HZV
Crystal structure of the zona pellucida module of human endoglin/CD105
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 125.160, 125.160, 88.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.504 - 2.700 |
R-factor | 0.234 |
Rwork | 0.232 |
R-free | 0.27200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3sex |
RMSD bond length | 0.002 |
RMSD bond angle | 0.534 |
Data reduction software | XDS ((Jun 17, 2015)) |
Data scaling software | XSCALE ((Jun 17, 2015)) |
Phasing software | PHASER ((2.5.6)) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.510 | 2.760 |
High resolution limit [Å] | 2.690 | 2.690 |
Rmerge | 0.675 | |
Number of reflections | 21583 | |
<I/σ(I)> | 9.43 | 0.99 |
Completeness [%] | 97.9 | 85.4 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 11.5% equal mixture of MPD, PEG 1000 and PEG 3350 (1:1:1), MES/imidazole mix |