5HZJ
Crystal structure of photoinhibitable Intersectin1 containing wildtype LOV2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97903 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.650, 111.170, 88.190 |
Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.059 | 0.453 |
Number of reflections | 35551 | |
<I/σ(I)> | 12.5 | 2.6 |
Completeness [%] | 97.0 | 97.2 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M HEPES pH 7.5, 9% (w/v) PEG 8000, 9% (v/v) ethylene glycol |