5HYI
Glycosylated, disulfide-linked Hole-Hole Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.282, 105.658, 134.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.150 - 2.900 |
| R-factor | 0.1779 |
| Rwork | 0.175 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.134 | |
| Number of reflections | 33226 | |
| <I/σ(I)> | 9.4 | 1.4 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 20% PEG8000, 0.1 M phosphate-citrate, 0.2 M sodium chloride, 0.55% n-dodecyl-N,N-dimethylamine-N-oxide |
