5HYE
Glycosylated Knob-Knob Fc fragment (P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.035, 81.624, 136.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.170 - 1.890 |
| R-factor | 0.2267 |
| Rwork | 0.224 |
| R-free | 0.27473 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.159 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 44608 | |
| <I/σ(I)> | 20.2 | 1.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1.0 M ammonium phosphate, 0.1 M citrate, 0.2 M sodium chloride |
