5HY9
Glycosylated, disulfide-linked Knob-into-Hole Fc fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 151.813, 151.813, 113.869 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.590 - 2.700 |
| R-factor | 0.21531 |
| Rwork | 0.212 |
| R-free | 0.27181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l6x |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.131 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.690 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 21747 | |
| <I/σ(I)> | 22.2 | 1.5 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 18.4 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.5 M sodium chloride, 0.1 M acetate |
