5HXA
Crystal structure of an UDP-forming alpha, alpha-terhalose-phosphate synthase from Burkholderia xenovorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 171.180, 171.180, 95.150 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.280 - 2.000 |
R-factor | 0.158 |
Rwork | 0.157 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wtx |
RMSD bond length | 0.007 |
RMSD bond angle | 0.766 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 2.050 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.073 | 0.454 |
Number of reflections | 55408 | |
<I/σ(I)> | 18.26 | 4.52 |
Completeness [%] | 99.4 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | MCSG1 A8 (267517a8): 100mM Tris:HCl pH7.0, 20% (w/v) PEG2000MME, 3mM UDP-glucose, 3mM glucose-6-phosphate, protein conc. 20mg/mL, 20% ethylene glycol cryoprotectant, puck hnk1-2 |