5HVW
Monomeric IgG4 Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 121.379, 121.379, 99.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.000 - 1.950 |
| R-factor | 0.21165 |
| Rwork | 0.209 |
| R-free | 0.25824 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.064 | 0.718 |
| Number of reflections | 19638 | |
| <I/σ(I)> | 33.1 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 9 mM ZnCl2, 90 mM Hepes, pH 7.0, 18% PEG 6000 (w/v) |
